{"product_id":"bacterial-secretion-systems-methods-and-protocols-9781071634479","title":"Bacterial Secretion Systems: Methods and Protocols","description":"\u003cp\u003e • Author(s): Laure Journet\u003cbr\u003e • Publisher: Springer\u003cbr\u003e • Publisher Imprint: Humana\u003cbr\u003e • BISAC: Life Sciences - Microbiology\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFrom the Back Cover\u003c\/b\u003e\u003cbr\u003eThis second edition details new and updated protocols that cover techniques used to study secretion systems. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and\/or secretion, identifying secreted effectors as well as using reporters to follow effector transport. Written in the highly successful \u003ci\u003eMethods in Molecular Biology\u003c\/i\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003eAuthoritative and cutting-edge, \u003ci\u003eBacterial Secretion Systems: Methods and Protocols- Second Edition \u003c\/i\u003eaims to be a useful andpractical guide to new researchers and experts looking to expand their knowledge. \u003c\/p\u003e","brand":"Springer","offers":[{"title":"Paperback","offer_id":45279896273047,"sku":"9781071634479","price":13098.0,"currency_code":"INR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0666\/3471\/1191\/files\/9781071634479.webp?v=1769294845","url":"https:\/\/atlanticbooks.com\/products\/bacterial-secretion-systems-methods-and-protocols-9781071634479","provider":"Atlantic Books","version":"1.0","type":"link"}