{"product_id":"calcium-techniques-a-laboratory-manual-9781936113583","title":"Calcium Techniques: A Laboratory Manual","description":"\u003cp\u003e • Author(s): Jan B. Parys | Martin Bootman | David I. Yule\u003cbr\u003e • Publisher: Cold Spring Harbor Laboratory Press\u003cbr\u003e • Publisher Imprint: Cold Spring Harbor Laboratory Press\u003cbr\u003e • BISAC: Life Sciences - Biochemistry\u003c\/p\u003e\u003cp\u003eLife begins with a surge of calcium ions (Ca2+) at fertilization, and thereafter, Ca2+ signaling influences nearly every aspect of mammalian development and physiology, from gene expression and cell proliferation to muscle contraction and nerve impulses. To create spatiotemporally distinct Ca2+ signals, cells use a variety of mechanisms to recognize, transport, and buffer Ca2+. Thus, a diverse range of reliable experimental techniques is necessary to study the movement of Ca2+ and the various effectors involved. \u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eThis laboratory manual provides step-by-step protocols for studying many facets of Ca2+ signaling, as well as background information on the principles and applications of the techniques. Contributors discuss how to use fluorescent, luminescent, and genetically encoded Ca2+ probes in conjunction with state-of-the-art imaging modalities to characterize Ca2+ signals. Electrophysiological measurements of Ca2+ channel activity are described, as are radioactive Ca2+ flux assays and methods to investigate signaling mediated by specific Ca2+-mobilizing messengers (IP3, cADPR, and NAADP). Techniques to modulate and suppress intra- and intercellular signals are also provided. Each protocol is complete with a list of required materials, detailed recipes for media and reagents, and troubleshooting advice. \u003cp\u003e\u003c\/p\u003eSpecific chapters are devoted to Ca2+ signaling techniques in non-mammalian systems, such as plants, yeast, zebrafish, and Xenopus. Methods for assessing Ca2+-binding kinetics and strategies for developing mathematical models of Ca2+ signaling are also included. Thus, this manual is a comprehensive laboratory resource for biochemists, cell and developmental biologists, and physiologists who are using or looking to expand their repertoire of Ca2+ techniques.","brand":"Cold Spring Harbor Laboratory Press","offers":[{"title":"Paperback","offer_id":47574898638999,"sku":"9781936113583","price":3203.0,"currency_code":"INR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0666\/3471\/1191\/files\/9781936113583.webp?v=1774896631","url":"https:\/\/atlanticbooks.com\/products\/calcium-techniques-a-laboratory-manual-9781936113583","provider":"Atlantic Books","version":"1.0","type":"link"}