{"product_id":"digital-pcr-methods-and-protocols-9781071647660","title":"Digital PCR: Methods and Protocols","description":"\u003cp\u003e • Author(s): Guillaume Pavlovic\u003cbr\u003e • Publisher: Humana\u003cbr\u003e • Publisher Imprint: Humana\u003cbr\u003e • BISAC: Life Sciences - Biology\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003eThis detailed volume explores digital polymerase chain reaction (dPCR), a third generation PCR technology that enables absolute quantification by counting positive and negative partitions after endpoint amplification. The book aims to provide an overview of the various possibilities currently available, both in terms of instrumentation and applications. The selected protocols cover a wide range of uses, including fundamental research, preclinical studies, human applications, and regulatory contexts. Additionally, protocols that further extend potential applications, such as protein detection, the development of custom dPCR systems, and alternative PCR strategies like hpPCR, which, while not digital, are relevant in similar contexts, are covered. Written for the highly successful \u003cem\u003eMethods in Molecular Biology\u003c\/em\u003e series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible protocols, and tips for troubleshooting and avoiding known pitfalls. \u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003eAuthoritative and practical, \u003cem\u003eDigital PCR: Methods and Protocols\u003c\/em\u003e serves as an ideal guide to this mature technology that will continue to enhance target quantification, notably through its multiplexing capabilities, tolerance to inhibitors, and increased precision.\u003c\/p\u003e","brand":"Atlantic Books","offers":[{"title":"Hardcover","offer_id":46437706793111,"sku":"9781071647660","price":20305.0,"currency_code":"INR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0666\/3471\/1191\/files\/9781071647660.webp?v=1769105113","url":"https:\/\/atlanticbooks.com\/products\/digital-pcr-methods-and-protocols-9781071647660","provider":"Atlantic Books","version":"1.0","type":"link"}