{"product_id":"directed-evolution-methods-and-protocols-9781071621547","title":"Directed Evolution: Methods and Protocols","description":"\u003cp\u003e • Author(s): Andrew Currin\u003cbr\u003e • Publisher: Springer\u003cbr\u003e • Publisher Imprint: Humana\u003cbr\u003e • BISAC: Biotechnology\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFrom the Back Cover\u003c\/b\u003e\u003cbr\u003eThis volume explores the latest techniques used by researchers to study directed evolution (DE) at each stage of the Design-Build-Test-Learn cycle. Chapters in this book cover topics such as designing overlap extension PCR primers for protein mutagenesis; antha-guided automation of Darwin assembly for the construction of bespoke gene libraries; rapid cloning of random mutagenesis libraries using PTO-Quickstep; and DE of glycosyltransferases by a single-cell screening method. Written in the highly successful \u003ci\u003eMethods in Molecular Biology\u003c\/i\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. \u003cbr\u003eCutting-edge and comprehensive, \u003ci\u003eDirected Evolution: Methods and Protocols\u003c\/i\u003e is a valuable resource for scientists and researchers who are interested in learning more about this field and incorporating these studies into new experimental workflows.\u003cbr\u003e\u003c\/p\u003e","brand":"Springer","offers":[{"title":"Paperback","offer_id":45280065126551,"sku":"9781071621547","price":11279.0,"currency_code":"INR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0666\/3471\/1191\/files\/9781071621547.webp?v=1769295276","url":"https:\/\/atlanticbooks.com\/products\/directed-evolution-methods-and-protocols-9781071621547","provider":"Atlantic Books","version":"1.0","type":"link"}