{"product_id":"dna-replicons-next-generation-vaccines-against-allergy-9783836495554","title":"DNA Replicons - Next-generation Vaccines against Allergy","description":"\u003cp\u003e • Author(s): Maximilian Gabler\u003cbr\u003e • Publisher: VDM Verlag Dr. Mueller E.K.\u003cbr\u003e • Publisher Imprint: VDM Verlag Dr. Mueller E.K.\u003cbr\u003e • BISAC: Genetics\u003c\/p\u003e\u003cp\u003eIn this study, DNA vaccines based on alphaviral replicases (DNA replicons) were evaluated with respect to their protective and therapeutic capacity for treatment of type I allergy. DNA replicons display specific danger signals and offer an excellent safety profile based on apoptotic elimination of transfected host cells thereby preventing undesirable long-term expression of the transfected gene. Using the model allergen beta-Galactosidase in a protective approach, the results showed that a replicase-based DNA vaccine can induce a comparable immune response at a more than 1000-fold lower dose than a conventional DNA vaccine. DNA replicon doses as low as 10 ng significantly prevented from IgE induction by subcutaneous protein sensitization. Furthermore, in a therapeutic approach with the same construct, DNA replicons balanced an ongoing allergic response. These data were confirmed with a DNA replicon encoding the clinically relevant timothy grass pollen allergen Phl p 5. In addition, lung pathology could be prevented by pre-vaccination with DNA replicons as measured by the magnitude of eosinophilia, goblet cell hyperplasia, IL-5 production and proliferation of endothelial cells.\u003c\/p\u003e","brand":"VDM Verlag Dr. Mueller E.K.","offers":[{"title":"Paperback","offer_id":46887545438359,"sku":"9783836495554","price":5437.0,"currency_code":"INR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0666\/3471\/1191\/files\/9783836495554.webp?v=1770256359","url":"https:\/\/atlanticbooks.com\/products\/dna-replicons-next-generation-vaccines-against-allergy-9783836495554","provider":"Atlantic Books","version":"1.0","type":"link"}