{"product_id":"e-coli-probiotic-for-piglet-diarrhoea-9783639026276","title":"E.coli: Probiotic for Piglet Diarrhoea","description":"\u003cp\u003e • Author(s): Amit Setia | Denis O. Krause\u003cbr\u003e • Publisher: VDM Verlag Dr. Mueller E.K.\u003cbr\u003e • Publisher Imprint: VDM Verlag Dr. Mueller E.K.\u003cbr\u003e • BISAC: Veterinary Medicine - General\u003c\/p\u003e\u003cp\u003eAim of this study was to select environmental E. coli isolates that pro-duced colicins against the swine pathogen E. coli K88+. In initial evaluation using a modified plate method with 18 colicinogenic E. coli constructs, colicins E3, E4, E5, E9, Ia, K and N were found to possess inhibitory activity against 12 ETEC K88+ strains. A total of 463 environmental isolates from cattle rumen, cattle feces, pig feces and hog manure-amended soil were screened for colicin production by a modified plate test. Further, colicinogenic isolates were screened for five toxin genes LT, STa, STb, VT1 and VT2 as well as K88 (F4) fimbriae using PCR reactions. Fourteen non-pathogenic isolates were subjected to characterization of colicin genes by PCR using 9 new primer sequences, antibiotic susceptibilities and substrate utilization. Two potential probiotic strains of E. coli, UM-2 and UM-7 which produced colicins that could utilize potato starch and inulin were selected for in-vitro competition with E. coli K88+ strain 2-12. In vitro competition between the synbiotics and E. coli K88+ revealed inhibition of E. coli K88+. Animal testing of these strains is currently being undertaken.\u003c\/p\u003e","brand":"VDM Verlag Dr. Mueller E.K.","offers":[{"title":"Paperback","offer_id":46887401455767,"sku":"9783639026276","price":4517.0,"currency_code":"INR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0666\/3471\/1191\/files\/9783639026276.webp?v=1770255814","url":"https:\/\/atlanticbooks.com\/products\/e-coli-probiotic-for-piglet-diarrhoea-9783639026276","provider":"Atlantic Books","version":"1.0","type":"link"}