{"product_id":"enzyme-mediated-ligation-methods-9781493995455","title":"Enzyme-Mediated Ligation Methods","description":"\u003cp\u003e • Author(s): Timo Nuijens\u003cbr\u003e • Publisher: Springer\u003cbr\u003e • Publisher Imprint: Humana\u003cbr\u003e • BISAC: Biotechnology\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFrom the Back Cover\u003c\/b\u003e\u003cbr\u003eThis volume discusses different enzyme-catalyzed ligation methodologies for a variety of different chemical transformations. This book wants readers to view enzymes as a powerful tool in both academic and industrial research. Chapters in this book cover topics such as sortase A-mediated generation of site-specifically conjugated antibody-drug conjugates; omniligase-catalyzed inter- and intramolecular ligation; ligation catalyzed by microbial transglutaminase; peptide cyclization mediated by cyanobactin macrocyclases, butelase 1 and sortase A; using BioID as a tool for protein proximity labeling in living cells; and inducible, selective labeling of proteins via enzymatic oxidation of tyrosine. Written in the highly successful \u003ci\u003eMethods in Molecular Biology\u003c\/i\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. \u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eCutting-edge and thorough, \u003ci\u003eEnzyme-Mediated Ligation Methods\u003c\/i\u003e is a valuable resource for students and scientists from different disciplines who are interested in using enzymatic strategies to answer their research questions. \u003cbr\u003e","brand":"Springer","offers":[{"title":"Hardcover","offer_id":45280043368599,"sku":"9781493995455","price":11752.0,"currency_code":"INR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0666\/3471\/1191\/files\/9781493995455.webp?v=1769295221","url":"https:\/\/atlanticbooks.com\/products\/enzyme-mediated-ligation-methods-9781493995455","provider":"Atlantic Books","version":"1.0","type":"link"}