{"product_id":"exon-skipping-methods-and-protocols-9781493962068","title":"Exon Skipping: Methods and Protocols","description":"\u003cp\u003e • Author(s): Annemieke Aartsma-Rus\u003cbr\u003e • Publisher: Springer\u003cbr\u003e • Publisher Imprint: Humana\u003cbr\u003e • BISAC: Genetics\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFrom the Back Cover\u003c\/b\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\"Next generation\" sequencing techniques allow for more detailed analysis of exons and introns in multiple genes at the same time. This will reveal many mutations that potentially lead to exon skipping. To functionally test these a lot can be achieved with a limited set of protocols, while for the intentional induction of exon skipping different tools and target genes are involved and the translational path from \u003ci\u003ein vitro\u003c\/i\u003e splicing to \u003ci\u003ein vivo\u003c\/i\u003e tests in animal models requiring a more extensive set of protocols. \u003ci\u003eExon Skipping: Methods and Protocols\u003c\/i\u003e provides scientist with a comprehensive guide to many of the methods and techniques used for exon skipping, such as methods on how to discriminate \"real polymorphisms\" from mutations that affect splicing. Written in the highly successful \u003ci\u003eMethods in Molecular Biology(TM)\u003c\/i\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.\u003c\/p\u003e \u003cp\u003eAuthoritative and practical\u003ci\u003e Exon Skipping: Methods and Protocols\u003c\/i\u003e seeks to aid scientists in the continuing study of exon skipping.\u003c\/p\u003e","brand":"Springer","offers":[{"title":"Paperback","offer_id":45273821937815,"sku":"9781493962068","price":9460.0,"currency_code":"INR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0666\/3471\/1191\/files\/9781493962068.webp?v=1769237721","url":"https:\/\/atlanticbooks.com\/products\/exon-skipping-methods-and-protocols-9781493962068","provider":"Atlantic Books","version":"1.0","type":"link"}