{"product_id":"golden-gate-cloning-methods-and-protocols-9781071642221","title":"Golden Gate Cloning: Methods and Protocols","description":"\u003cp\u003e • Author(s): Daniel Schindler\u003cbr\u003e • Publisher: Humana\u003cbr\u003e • Publisher Imprint: Humana\u003cbr\u003e • BISAC: Life Sciences - Genetics \u0026amp; Genomics\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003cspan\u003eThis volume provides an overview of the latest protocols that cover the full range of \u003c\/span\u003e\u003cspan class=\"a-text-italic\"\u003ein silico\u003c\/span\u003e\u003cspan\u003e, \u003c\/span\u003e\u003cspan class=\"a-text-italic\"\u003ein vitro\u003c\/span\u003e\u003cspan\u003e, and \u003c\/span\u003e\u003cspan class=\"a-text-italic\"\u003ein vivo\u003c\/span\u003e\u003cspan\u003e approaches to help researchers plan, design, and perform Golden Gate cloning experiments. The chapters in this book cover topics such as Golden Gate cloning of standardized parts; modular DNA construct design for high-throughput Golden Gate Assembly; Golden Gate cloning of Synthetic CRISPR RNA spacer sequences; Golden Gate cloning in Actinobacteria; and YeastFab cloning of toxic genes and protein expression optimization in yeast. Written in the highly successful \u003c\/span\u003e\u003cspan class=\"a-text-italic\"\u003eMethods in Molecular Biology\u003c\/span\u003e\u003cspan\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.\u003cbr\u003e \u003cbr\u003e Cutting-edge and thorough, \u003c\/span\u003e\u003cspan class=\"a-text-italic\"\u003eGolden Gate Cloning: Methods and Protocols \u003c\/span\u003e\u003cspan\u003eis a valuable resource for all researchers interested in learning more about this important and developing field.\u003cbr\u003e \u003cbr\u003e Chapter 3 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.\u003c\/span\u003e\u003c\/p\u003e","brand":"Humana","offers":[{"title":"Paperback","offer_id":47854026326167,"sku":"9781071642221","price":10408.0,"currency_code":"INR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0666\/3471\/1191\/files\/9781071642221.webp?v=1780038163","url":"https:\/\/atlanticbooks.com\/products\/golden-gate-cloning-methods-and-protocols-9781071642221","provider":"Atlantic Books","version":"1.0","type":"link"}