{"product_id":"hplc-uv-method-development-for-fixed-dose-pharmaceutical-formulations-9786209779718","title":"HPLC-UV Method development for Fixed Dose Pharmaceutical Formulations","description":"\u003cp\u003e • Author(s): Sonia Yadav | Anunny Sharma | Sushma Maratha\u003cbr\u003e • Publisher: LAP Lambert Academic Publishing\u003cbr\u003e • Publisher Imprint: LAP Lambert Academic Publishing\u003cbr\u003e • BISAC: Pharmacology\u003c\/p\u003e\u003cp\u003eEstimation of imeglimin hydrochloride in human plasma by HPLC-UV. The estimation of drugs by RP-HPLC (reverse phase chromatography) was chosen since it is advised for use with ionic and mild to non-polar compounds. Simple, specific, and superior in terms of efficiency, stability, and reproducibility is reverse phase chromatography. For the separation of imeglimin, the C18 column 250 x 4.6 mm, 5  m particle size was chosen. In the mobile phase, various solvent solutions were tested and combined for optimum performance. Imeglimin hydrochloride (4  g\/ml) in buffer, pH 4 for Acetonitrile: Water (70:30), a concentration range (8-40 and 0.5-4  g\/ml) displaying excellent peak and a sizable resolution level. Having the mobile phase run at 1ml\/min, photodiode array detectors were used to detect both analytes at 215 nm. The method was validated in different concentration ranges in human plasma. Imeglimin inter and intra-run precision was measured at less than 3.60% and had an accuracy of less than 1.869%. imeglimin in human plasma was validated by linearity, recovery matrix, and stability. The retention time of IMEG is 4.8 minutes in plasma. Suggested method denotes that it is cost-effective\u003c\/p\u003e","brand":"LAP Lambert Academic Publishing","offers":[{"title":"Paperback","offer_id":47776201605271,"sku":"9786209779718","price":5022.0,"currency_code":"INR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0666\/3471\/1191\/files\/9786209779718.webp?v=1777994883","url":"https:\/\/atlanticbooks.com\/products\/hplc-uv-method-development-for-fixed-dose-pharmaceutical-formulations-9786209779718","provider":"Atlantic Books","version":"1.0","type":"link"}