{"product_id":"photoswitching-proteins-methods-and-protocols-9781071607572","title":"Photoswitching Proteins: Methods and Protocols","description":"\u003cp\u003e • Author(s): Dominik Niopek\u003cbr\u003e • Publisher: Springer\u003cbr\u003e • Publisher Imprint: Humana\u003cbr\u003e • BISAC: Neuroscience\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFrom the Back Cover\u003c\/b\u003e\u003cbr\u003eThis volume provides cutting edge protocols for using optogenetic and opto-chemical techniques across a wide spectrum of applications. Chapters guide readers through methods in neuro-optogenetics, including methods to dissect local and long-range connectivity ex vivo, investigate G-protein coupled receptors, study astrocyte-neuron networks in vitro and in vivo, employ upconversion nanoparticles for transcranial optogenetic stimulation, and use Adeno-associated virus (AAV) for delivery of optogenetic constructs. At the same time, this book covers a range of methods for non-neuronal optogenetics, including various applications of light-oxygen-voltage domains or cryptochromes for control of nucleocytoplasmic transport, transgene expression and chromatin recruitment. The book further presents protocols for traceless light-control of gene expression with melanopsin, light-inducible cAMP production, use of light-regulated receptor tyrosine kinases (Opto-RTKs), synthesis of light-dependent biomaterials, and highlights emerging tools at the intersection of optogenetics and CRISPR. Written in the highly successful \u003ci\u003eMethods in Molecular Biology\u003c\/i\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003eAuthoritative and cutting-edge, \u003ci\u003ePhotoswitching Proteins: Methods and Protocols \u003c\/i\u003eaims to provide a comprehensive list of methods and tools to investigate highly dynamic pathways and behaviors in cells and animals using optogenetics.\u003c\/p\u003e","brand":"Springer","offers":[{"title":"Paperback","offer_id":45280141901975,"sku":"9781071607572","price":8005.0,"currency_code":"INR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0666\/3471\/1191\/files\/9781071607572.webp?v=1769295464","url":"https:\/\/atlanticbooks.com\/products\/photoswitching-proteins-methods-and-protocols-9781071607572","provider":"Atlantic Books","version":"1.0","type":"link"}