{"product_id":"proximity-labeling-methods-and-protocols-9781493995363","title":"Proximity Labeling: Methods and Protocols","description":"\u003cp\u003e • Author(s): Murat Sunbul\u003cbr\u003e • Publisher: Springer\u003cbr\u003e • Publisher Imprint: Humana\u003cbr\u003e • BISAC: Life Sciences - Biochemistry\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFrom the Back Cover\u003c\/b\u003e\u003cbr\u003eThis book provides detailed protocols and untold tips and tricks regarding the most well-known examples of proximity labeling methods, in which the protein of interest is genetically fused to or labeled with an enzyme that can generate short-lived reactive species to non-specifically label molecules within a certain radius of up to twenty nanometers. Beginning with peroxidase-based proximity labeling methods, the volume continues with BioID, proximity labeling methods that describe the proximity ligation assay to detect RNA-DNA interactions, UV cross-linking to demonstrate RNA-protein interactions, and how chemical and enzymatic reactivities can be improved upon DNA-DNA and protein-protein interactions, as well as \"proximity-induced self-labeling,\" where the radius of labeling is zero. Written for the highly successful \u003ci\u003eMethods in Molecular Biology\u003c\/i\u003e series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. \u003cbr\u003eAuthoritative and practical, \u003ci\u003eProximity Labeling: Methods and Protocols\u003c\/i\u003e serves as an ideal guide for researchers exploring the crucial roles that proximity-driven reactions play in biological systems.\u003c\/p\u003e","brand":"Springer","offers":[{"title":"Hardcover","offer_id":45279481692311,"sku":"9781493995363","price":14690.0,"currency_code":"INR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0666\/3471\/1191\/files\/9781493995363.webp?v=1769293696","url":"https:\/\/atlanticbooks.com\/products\/proximity-labeling-methods-and-protocols-9781493995363","provider":"Atlantic Books","version":"1.0","type":"link"}