{"product_id":"two-hybrid-systems-methods-and-protocols-9781493993109","title":"Two-Hybrid Systems: Methods and Protocols","description":"\u003cp\u003e • Author(s): Luis Oñate-Sánchez\u003cbr\u003e • Publisher: Springer\u003cbr\u003e • Publisher Imprint: Humana\u003cbr\u003e • BISAC: Life Sciences - Biochemistry\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFrom the Back Cover\u003c\/b\u003e\u003cbr\u003eThis detailed book explores the numerous applications of the yeast two-hybrid (Y2H) system, one of the most commonly used methods to detect protein-protein interactions. Beginning with an overview, the volume then continues by elucidating different methodologies to detect protein-protein interactions in yeast nucleus, membrane, cytoplasm, and bacteria, computational analyses of protein interaction networks, technical variations in yeast one-hybrid (Y1H) systems together with classical and more elaborated yeast methods to detect protein-DNA interactions, as well as protocols to analyze ternary protein interactions and RNA-protein and ligand-protein interactions. The book concludes with a section on methods in plant and mammalian cells that can be used to identify novel protein-protein and protein-DNA interactions and test (qualitatively and\/or quantitatively) those observed in yeast. Written for the highly successful \u003ci\u003eMethods in Molecular Biology\u003c\/i\u003e series, chapters include the kind of detail and key implementation advice that leads to excellent results in the lab. \u003cbr\u003eAuthoritative and practical, \u003ci\u003eTwo-Hybrid Systems: Methods and Protocols\u003c\/i\u003e serves as an ideal guide to the uses of this exceedingly valuable technique.\u003c\/p\u003e","brand":"Springer","offers":[{"title":"Paperback","offer_id":45279678726295,"sku":"9781493993109","price":12119.0,"currency_code":"INR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0666\/3471\/1191\/files\/9781493993109.webp?v=1769294247","url":"https:\/\/atlanticbooks.com\/products\/two-hybrid-systems-methods-and-protocols-9781493993109","provider":"Atlantic Books","version":"1.0","type":"link"}