{"product_id":"type-3-secretion-systems-methods-and-protocols-9781493982608","title":"Type 3 Secretion Systems: Methods and Protocols","description":"\u003cp\u003e • Author(s): Matthew L. Nilles\u003cbr\u003e • Publisher: Springer\u003cbr\u003e • Publisher Imprint: Humana\u003cbr\u003e • BISAC: Life Sciences - Microbiology\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFrom the Back Cover\u003c\/b\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003eThis volume discusses various basic and advanced methods and protocols that have been proven to be successful among certain bacterial species, or a family of species, in type III secretion systems (T3S system). The chapters in this book cover topics such as: site-directed mutagenesis and its application in studying the interactions of T3S components; use of transcriptional control to increase expression and secretion of heterologous proteins in T3S systems; fractionation techniques to examine effector translocation; detecting immune responses to T3S systems; mouse immunization with purified needle proteins from T3S systems and the characterization of the immune responses to these proteins; and detection of protein interactions in T3S systems using yeast 2-hybrid analysis. Written in the highly successful \u003ci\u003eMethods in Molecular Biology \u003c\/i\u003eseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. \u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e Practical and comprehensive, \u003ci\u003eType 3 Secretion Systems: Methods and Protocols\u003c\/i\u003e is a valuable resource for anyone interested in learning about the fascinating and ever-changing T3S systems.\u003cb\u003e \u003c\/b\u003e\u003cp\u003e\u003c\/p\u003e ","brand":"Springer","offers":[{"title":"Paperback","offer_id":45277139370135,"sku":"9781493982608","price":8005.0,"currency_code":"INR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0666\/3471\/1191\/files\/9781493982608.webp?v=1769287593","url":"https:\/\/atlanticbooks.com\/products\/type-3-secretion-systems-methods-and-protocols-9781493982608","provider":"Atlantic Books","version":"1.0","type":"link"}