{"product_id":"vascular-hyperpermeability-methods-and-protocols-9781071634318","title":"Vascular Hyperpermeability: Methods and Protocols","description":"\u003cp\u003e • Author(s): Binu Tharakan\u003cbr\u003e • Publisher: Springer\u003cbr\u003e • Publisher Imprint: Humana\u003cbr\u003e • BISAC: Research\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFrom the Back Cover\u003c\/b\u003e\u003cbr\u003eThis volume focuses on the latest research methods and techniques used by researchers to study vascular permeability\/hyperpermeability in a science laboratory setting. The chapters in this book cover topics such as determination of solute permeability of microvascular endothelial cell monolayers in vitro; evaluation of barrier integrity used in a two-layered microfluidic device mimicking the blood-brain barrier; isolation and culture of human umbilical vein endothelial cells; measurement of blood-brain barrier hyperpermeability using Evans Blue extravasation assay; lymphatic vascular permeability determined from direct measurements of solute flux; intravital imaging of leukocyte-endothelial interaction by intravital multiphoton microscopy; and evaluation of tight junction integrity in brain endothelial cells using confocal microscopy. Written in the highly successful \u003ci\u003eMethods in Molecular Biology\u003c\/i\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.\u003cbr\u003eThorough and cutting-edge, \u003ci\u003eVascular Hyperpermeability: Methods and Protocols\u003c\/i\u003e is a valuable resource that will aid novice researchers with creating new and affordable research projects, and for expert researchers to initiate new strategies and collaborations in their ongoing programs. \u003cbr\u003e\u003c\/p\u003e","brand":"Springer","offers":[{"title":"Paperback","offer_id":45280075415703,"sku":"9781071634318","price":8732.0,"currency_code":"INR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0666\/3471\/1191\/files\/9781071634318.webp?v=1769295305","url":"https:\/\/atlanticbooks.com\/products\/vascular-hyperpermeability-methods-and-protocols-9781071634318","provider":"Atlantic Books","version":"1.0","type":"link"}