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Molecular Analysis of DNA Rearrangements in the Immune System by Rolf Jessberger

Molecular Analysis of DNA Rearrangements in the Immune System

by Rolf Jessberger
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Book cover type: Paperback
  • ISBN13: 9783642501425
  • Binding: Paperback
  • Subject: N/A
  • Publisher: Springer
  • Publisher Imprint: Springer
  • Publication Date:
  • Pages: 224
  • Original Price: EUR 49.99
  • Language: English
  • Edition: Softcover Repri
  • Item Weight: 368 grams
  • BISAC Subject(s): General, Immunology, and Life Sciences / Molecular Biology

The vertebrate immune system is distinctive among defense systems of multicellular organisms. In addition to nonspecific immunity, it generates a randomized array of millions of antigen receptors (immunoglobulins and T-cell receptors). A subset of these receptors are critical for binding to invading microbes or biochemicals from them to tag the microbes for elimination. Three site-directed DNA modification processes are critical to this process in vertebrates. V(D)J recombination generates the array of exons that encode the antigen binding pockets. Recent work summarized in this volume describes the dissection of this process at the biochemical level. The mechanism of the reaction is now understood in considerable detail. The proteins that catalyze many steps of the process have now been identified by biochemical and genetic recon- stitution and by analysis of genetic mutants defective in V(D)J recombination. Class switch recombination is the process by which the variable domain exon of the heavy chain is changed from IgM to IgG, IgA. or IgE. Recent progress is described in the de- velopment of an extrachromosomal substrate assay system. Molecular genetic analysis of the process in transgenics is defining some of the cis sequence requirements. Biochemical assays for defining enzymatic components are also described. In addition to exciting progress in V(D)J recombination and class switch recombination, one chapter describes recent pro- gress in somatic hypermutation.

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