From the Back Cover
It is now the age of the specialized cell in culture, R. Ian Freshney wrote in the preface to the first edition of Culture of Epithelial Cells. Realizing the inadequacy of studying all types of cells at random in a culture, it became imperative for researchers to learn how unique cells perform under a wide variety of conditions. Now the Second Edition of Culture of Epithelial Cells--devoted to those cells that line the body, internally and externally, protecting surfaces and secreting or absorbing material--advances this ethic, providing completely revised, detailed descriptions of fundamental and practical aspects relating to the in vitro cultivation of disparate types of epithelia.
Each chapter of the Second Edition is devoted to a different type of epithelium--epidermal, mammary, cervical, prostatic, oral, intestinal, pulmonary, hepatic, and urothelial. R. Ian Freshney also contributes a final chapter on a number of epithelia not covered in the main chapters, presenting them together in review form with additional protocols. Other features of the Second Edition of Culture of Epithelial Cells include:
- Proven methods refined by the authors, all well-established researchers
- Alternate techniques coupled with critical comparative analyses
- Logical anatomical organization by tissue type
- Stepwise culture protocols conveniently highlighted
- New chapters on the culture of oral, alveolar, intestinal, and urothelial epithelium
- In-depth analysis of cell-cell interaction in skin
Displaying the clear, easy-to-follow style that has become a trademark of the Culture of Specialized Cells series, the Second Edition of Culture of Epithelial Cells remains an essential resource for researchers in cell and developmental biology, cancer biology, tissue engineering, biochemistry, and genetics, where the work requires the cultivation or manipulation of cells from the epithelium.
R. Ian Freshney gained a BSc (1960) and PhD (1964) from Glasgow University. He spent 1965 in Madison, Wisconsin, working on cell interaction and differentiation of embryonic mouse liver before returning to Glasgow to join the Beatson Institute for Cancer Research until 1981. At this time he transferred to Medical Oncology at Glasgow University, where he became a senior lecturer, teaching cell culture and tumor cell biology, with research interests in stromal interaction and differentiation in tumors. He is now an honorary senior research fellow at Glasgow University, focusing his attention on teaching, writing and editing in the field of cell culture.
Mary G. Freshney gained considerable tissue culture experience, initially as a technician with Dr. John Paul, and laterally as a scientific assistant working on hematopoietic stem cells at the Beatson Institute, where she had the major responsibility for the management of the cell culture facility.
