Effets du DON et du DOM-1 sur la fonction des cellules thécales ovariennes bovines
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La mycotoxine d�soxynival�nol (DON) et son m�tabolite, le d�soxy-DOM-1, ont des effets significatifs sur la fonction des cellules th�cales ovariennes bovines. L'objectif de cette �tude �tait d'identifier, par spectrom�trie de masse, les diff�rentes voies de signalisation impliqu�es dans le m�canisme d'action du DON et du DOM-1. M�thodes: des cellules th�cales ovariennes bovines ont �t� pr�lev�es sur des vaches adultes, quel que soit le stade du cycle oestral, et ont �t� cultiv�es � une densit� de 500 000 cellules viables dans 1 ml de milieu de McCoy pendant 5 jours. Les cellules ont ensuite �t� trait�es au cinqui�me jour de culture avec 1 ng/mL de DON ou de DOM-1 pendant 30 minutes, puis les prot�ines cellulaires totales ont �t� recueillies pour la spectrom�trie de masse. Les r�sultats de la spectrom�trie de masse ont montr� que le DON et le DOM-1 induisent tous deux une r�gulation � la hausse simultan�e de l'ERK1/2, de la MAPK14 (p38alpha) et de la MAPK13 (p38delta). La spectrom�trie de masse a �galement indiqu� que 94 peptides, tels que GNGT1, EDN1 et YWHAB, �taient r�gul�s � la hausse. Ils r�gulent principalement les voies de prolif�ration cellulaire et sont impliqu�s dans la biosynth�se des lipides et des glucides. N�anmoins, 255 peptides, tels que CALR3, PTGES3, RAD21, ACVR2B et TGFBR1, ont �t� r�gul�s � la baisse.
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