Methodenentwicklung für Amlodipin und Nevirapin mittels UV-Spektroskopie und HPTLC

Name: Methodenentwicklung für Amlodipin und Nevirapin mittels UV-Spektroskopie und HPTLC
Brand: Verlag Unser Wissen
SKU: 9786630054675
Price: 7340.0 INR
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by Sathis Kumar Dinakaran , Narayudu Yandamuri

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ISBN13: 9786630054675
Binding: Paperback
Subject: N/A
Publisher: Verlag Unser Wissen
Publisher Imprint: Verlag Unser Wissen
Publication Date: May 24, 2026
Pages: 92
Original Price: GBP 58.0
Language: German
Edition: N/A
Item Weight: 137 grams
BISAC Subject(s): Pharmacology

About The Book

Es wurden eine einfache, kosteng�nstige und genaue Absorptionsverh�ltnis-Methode in der UV-Spektroskopie sowie eine HPTLC-Methode zur gleichzeitigen Bestimmung von Amlodipinbesilat (AML) und Nevirapin (NEV) in Rohmaterial und Tabletten entwickelt. In der Spektroskopie wurde 0,1 M HCl als Verd�nnungsmittel verwendet, um AML und NEV zu l�sen. Die Absorptionen wurden bei 252 nm (isobestischer Punkt) und 295 nm (λmax von NEV) gemessen, die auf der Grundlage der �berlappenden Spektren von AML und NEV ausgew�hlt wurden. Der Linearit�tsbereich der UV-Methode lag bei 3-7 g/ml bei 252 nm f�r AML (r = 0,9995 0,0005) und bei 295 nm f�r NEV (r = 0,9985 0,0012). Die HPTLC-Methode wurde mit einer Silicagel-GF-TLC-Platte entwickelt. Als L�sungsmittel wurde Chloroform-Methanol-Eisessig (75,8:21,6:2,6) verwendet. Der Fleck nach dem Chromatogramm wurde im UV-Licht und in der Jodkammer identifiziert. Der Linearit�tsbereich der HPTLC-Methode lag bei 5-25 g/ml f�r AML (r = 0,9994) und bei 295 nm f�r NEV (r = 0,999). Zur Best�tigung der Genauigkeit der Methoden wurden Wiederfindungsstudien mit UV- und HPTLC-Methoden durchgef�hrt. Die Methoden wurden gem� den ICH-Richtlinien validiert. Die Methoden erwiesen sich als einfach, pr�zise, genau und schnell f�r die gleichzeitige Bestimmung von AML und NEV in der Bulk- und Tabletten-Darreichungsform.

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