Sviluppo di un metodo per l'analisi di amlodipina e nevirapina mediante spettroscopia UV e HPTLC

Name: Sviluppo di un metodo per l'analisi di amlodipina e nevirapina mediante spettroscopia UV e HPTLC
Brand: Edizioni Sapienza
SKU: 9786630062359
Price: 7340.0 INR
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by Sathis Kumar Dinakaran , Narayudu Yandamuri

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ISBN13: 9786630062359
Binding: Paperback
Subject: N/A
Publisher: Edizioni Sapienza
Publisher Imprint: Edizioni Sapienza
Publication Date: May 24, 2026
Pages: 92
Original Price: GBP 58.0
Language: Italian
Edition: N/A
Item Weight: 137 grams
BISAC Subject(s): Pharmacology

About The Book

Sono stati sviluppati un metodo semplice, economico e accurato basato sul rapporto di assorbimento nella spettroscopia UV e un metodo HPTLC per la determinazione simultanea di amlodipina besilato (AML) e nevirapina (NEV) in prodotti sfusi e in compresse. Nella spettroscopia, � stato utilizzato HCl 0,1 M come diluente per dissolvere AML e NEV. Gli assorbimenti sono stati osservati a 252 nm (punto isobestico) e 295 nm (λmax di NEV), selezionati in base alla sovrapposizione degli spettri di AML e NEV. L'intervallo di linearit� del metodo UV � risultato essere 3-7 g/ml a 252 nm per l'AML (r2=0,9995 0,0005) e a 295 nm per la NEV (r2=0,9985 0,0012). Il metodo HPTLC � stato sviluppato utilizzando una piastra TLC in gel di silice GF. Come diluente � stata utilizzata una miscela di cloroformio-metanolo-acido acetico glaciale (75,8:21,6:2,6). La macchia risultante dal cromatogramma � stata identificata mediante UV e camera di iodio. L'intervallo di linearit� del metodo HPTLC � risultato essere 5-25 g/ml per l'AML (r = 0,9994) e 295 nm per il NEV (r = 0,999). Sono stati effettuati studi di recupero dei metodi UV e HPTLC per confermare l'accuratezza dei metodi. I metodi sono stati convalidati secondo le linee guida ICH. I metodi si sono rivelati semplici, precisi, accurati e rapidi per la determinazione simultanea di AML e NEV nella forma farmaceutica sfusa e in compresse.

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