Caracterização molecular de bactérias produtoras de polihidroxibutirato

Name: Caracterização molecular de bactérias produtoras de polihidroxibutirato
Brand: Edicoes Nosso Conhecimento
SKU: 9786209997655
Price: 5319.0 INR
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by Swati Shalini

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ISBN13: 9786209997655
Binding: Paperback
Subject: N/A
Publisher: Edicoes Nosso Conhecimento
Publisher Imprint: Edicoes Nosso Conhecimento
Publication Date: May 13, 2026
Pages: 76
Original Price: GBP 42.0
Language: Portuguese
Edition: N/A
Item Weight: 114 grams
BISAC Subject(s): Life Sciences / Microbiology

About The Book

Os polihidroxibutiratos s�o biopol�meros com potencial para serem utilizados como pl�sticos biodegrad�veis, em substitui��o dos pl�sticos sint�ticos. A presente investiga��o foi realizada com o objetivo de isolar bact�rias produtoras de polihidroxibutirato e analisar a diversidade entre elas, relacionada com a sequ�ncia do gene da PHB sintase. As amostras de solo e �gua para o isolamento das bact�rias foram recolhidas em catorze fontes diferentes. De entre cem isolados bacterianos, quarenta revelaram-se produtores de PHB ap�s um rastreio inicial baseado em fluoresc�ncia em meio contendo azul do Nilo A. Ao testar diferentes combina��es de fontes de carbono e azoto, verificou-se que 2 g de glicose como fonte de carbono e 0,65 g de nitrato de pot�ssio eram as mais adequadas. Foram selecionadas as seis melhores estirpes acumuladoras de PHB e caracterizadas para a otimiza��o dos meios de produ��o de PHB. As estirpes selecionadas produziam 59-29% de PHB em rela��o ao peso seco do sedimento celular. Foram testadas diferentes combina��es de subprodutos agr�colas como fonte de carbono para uma produ��o de PHB economicamente vi�vel, tendo-se verificado que 3 g de mela�o constitu�am a melhor fonte de carbono para a produ��o de PHB (60% do peso seco das c�lulas). A amplifica��o por PCR foi realizada com primers espec�ficos do gene da PHB sintase, utilizando ADN gen�mico das estirpes selecionadas.

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