Detecção de espécies de Cryptosporidium em amostras de fezes
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Um total de 500 amostras de fezes foram submetidas a exame atrav�s de microscopia direta e m�todos de concentra��o. Destas, cinquenta e duas (10,4%) amostras revelaram-se positivas para Cryptosporidium parvum. Entre os m�todos de microscopia direta - montagem direta em fresco, colora��o negativa e colora��o de Ziehl-Neelsen modificada - apresentaram uma positividade de 22 (4,4%), 14 (2,8%) e 39 (7,8%), respetivamente. Ap�s a t�cnica de concentra��o com formol-�ter (FEC), a montagem em fresco, a colora��o negativa e a colora��o de Ziehl-Neelsen modificada foram positivas em 32 (6,4%), 37 (7,4%) e 52 (10,4%) casos, respetivamente. A montagem em fresco ap�s a t�cnica de Flota��o em Sal de Cloreto de S�dio Saturado (SSSF) foi positiva em apenas 6 (1,2%) casos. A colora��o de Ziehl-Neelsen modificada ap�s a t�cnica SSSF foi positiva em 19 (3,8%) casos, enquanto a colora��o negativa ap�s a t�cnica SSSF foi positiva em apenas 1 (0,2%) caso. A positividade para Cryptosporidium parvum foi 1,18 vezes superior nos homens em compara��o com as mulheres. Os resultados indicam que a colora��o de Ziehl-Neelsen modificada ap�s a t�cnica FEC para detetar Cryptosporidium parvum em amostras de fezes apresenta uma precis�o (p
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