Molekulare Charakterisierung von Polyhydroxybutyrat produzierenden Bakterien

Name: Molekulare Charakterisierung von Polyhydroxybutyrat produzierenden Bakterien
Brand: Verlag Unser Wissen
SKU: 9786209987410
Price: 5319.0 INR
Availability: InStock

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ISBN13: 9786209987410
Binding: Paperback
Subject: N/A
Publisher: Verlag Unser Wissen
Publisher Imprint: Verlag Unser Wissen
Publication Date: May 13, 2026
Pages: 76
Original Price: GBP 42.0
Language: German
Edition: N/A
Item Weight: 114 grams
BISAC Subject(s): Life Sciences / Microbiology

About The Book

Polyhydroxybutyrate sind Biopolymere und bieten das Potenzial, als biologisch abbaubare Kunststoffe als Ersatz f�r synthetische Kunststoffe eingesetzt zu werden. Die vorliegende Untersuchung wurde durchgef�hrt, um Polyhydroxybutyrat produzierende Bakterien zu isolieren und deren Diversit�t in Bezug auf die Sequenz des PHB-Synthase-Gens zu analysieren. Die Boden- und Wasserproben f�r die Isolierung der Bakterien wurden aus vierzehn verschiedenen Quellen entnommen. Von hundert Bakterienisolaten erwiesen sich nach einem ersten fluoreszenzbasierten Screening auf einem Nile-Blue-A-haltigen Medium vierzig als PHB-produzierend. Durch das Ausprobieren verschiedener Kombinationen von Kohlenstoff- und Stickstoffquellen erwiesen sich 2 g Glukose als Kohlenstoffquelle und 0,65 g Kaliumnitrat als am besten geeignet. Sechs der besten PHB-akkumulierenden St�mme wurden ausgew�hlt und zur Optimierung der PHB-Produktionsmedien charakterisiert. Die ausgew�hlten St�mme produzierten 59-29 % PHB, bezogen auf das Trockengewicht des Zellpellets. Verschiedene Kombinationen von landwirtschaftlichen Nebenprodukten wurden als Kohlenstoffquelle f�r eine kosteneffiziente PHB-Produktion getestet, wobei sich 3 g Melasse als beste Kohlenstoffquelle f�r die PHB-Produktion erwiesen (60 % des Zell-Trockengewichts). Die PCR-Amplifikation wurde mit PHB-Synthase-genspezifischen Primern an genomischer DNA der ausgew�hlten St�mme durchgef�hrt.

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