Produzione di L-glutaminasi da un ceppo isolato dalle mangrovie
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Gli actinomiceti sono batteri filamentosi aerobici gram-positivi con un elevato contenuto di G+C (guanina + citosina) che formano spore asessuali e sono ampiamente diffusi sia negli habitat terrestri che in quelli acquatici. Costituiscono la principale fonte di antibiotici clinicamente importanti, la maggior parte dei quali � troppo complessa per essere sintetizzata mediante chimica combinatoria. La L-glutaminasi � un enzima prodotto da vari microrganismi che viene utilizzato per il trattamento della leucemia, come esaltatore di sapidit� e come sensore enzimatico. Anche se l'attivit� della L-glutaminasi � stata segnalata in vari microrganismi, la L-glutaminasi proveniente dagli actinomiceti � molto scarsa. Le mangrovie sono l'ecosistema pi� produttivo e inesplorato che copre circa un quarto delle coste mondiali con un'elevata diversit� di organismi rigogliosi. Recentemente, il tasso di isolamento di nuovi composti bioattivi da microrganismi nelle foreste di mangrovie � aumentato enormemente. Il presente studio ha evidenziato l'ambiente delle mangrovie come potenziale fonte di actinomiceti efficienti nella produzione di L-glutaminasi. � stata esaminata la produzione di L-glutaminasi da ceppi sia selvatici che mutanti mediante fermentazione in fase solida. Le condizioni di fermentazione sono state ottimizzate per massimizzare la produzione dell'enzima
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