Name: Neutrophil: Methods and Protocols
Brand: Springer
SKU: 9781071601563
Price: 8365.0 INR
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Neutrophil: Methods and Protocols

by Mark T. Quinn

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ISBN13: 9781071601563
Binding: Paperback
Subject: N/A
Publisher: Springer
Publisher Imprint: Humana
Publication Date: November 14, 2020
Pages: 470
Original Price: EUR 119.99
Language: English
Edition: 2020
Item Weight: 907 grams
BISAC Subject(s): Immunology, Life Sciences / Molecular Biology, and Life Sciences / Cell Biology

About The Book

Neutrophils, the most abundant white cells in humans, serve as the primary cellular defense against infection. This volume provides a concise set of protocols for assessing basic neutrophil functions and investigating specialized areas in neutrophil biology. Each of the protocols is written by leading researchers in the field and includes hints for success, as well as guidance for troubleshooting. In the third edition of Neutrophils: Methods and Protocols, chapters retained from previous editions have been have been updated and include many new approaches. In addition, the Third Edition contains a number of new chapters that were not included in the first or second editions. Part I contains overviews of neutrophil biology, function, and disorders. Part II describes commonly used methods to isolate neutrophils from humans and other animal species. Part III details methods for investigating chemotaxis, transmigration, phagocytosis, and bactericidal activity. Part IV includes protocols that measure neutrophil apoptosis, calcium signal transduction, degranulation and detection of cytoplasmic granules, gene expression, transcription factors, and apoptosis. Part V provides multiple assays for measuring production of intracellular and/or extracellular reactive oxygen species, and a method that details use of the cell-free NADPH oxidase assay, an iconic assay for studies of the neutrophil NADPH oxidase. Part VI provides chapters that describe how to analyze formation and function of neutrophil extracellular traps. Neutrophil Methods and Protocols: Third Edition is a comprehensive source for detailed explanations and applications of the most modern methodological advances in neutrophil biology. Both basic scientists and clinicians will find a collection of this caliber to be an invaluable aid in their work with neutrophils.

Features

- Provides neutrophil protocols that are broadly applicable to leukocyte biology

- Includes protocols for isolation of neutrophils from humans and animal species

- Presents an interesting and informative overview of the field of neutrophil biology

- Facilitates a better understanding of the role of neutrophils in host defense

Readership

Molecular and Cell Biologists, Immunologists, Infectious Disease Specialists, Microbiologists

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