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Application of DNA Microarray Technology for Wastewater Analysis by F. Berthiaume, R. Brousseau, K. Lemarchand

Application of DNA Microarray Technology for Wastewater Analysis

by F. Berthiaume , R. Brousseau , K. Lemarchand
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Book cover type: Paperback
  • ISBN13: 9781843397274
  • Binding: Paperback
  • Subject: N/A
  • Publisher: IWA Publishing (Intl Water Assoc)
  • Publisher Imprint: IWA Publishing (Intl Water Assoc)
  • Publication Date:
  • Pages: 112
  • Original Price: GBP 145.0
  • Language: English
  • Edition: N/A
  • Item Weight: 273 grams
  • BISAC Subject(s): Chemical & Biochemical

Deoxyribonucleic acid (DNA) microarrays are widely used for differential expression studies and for detection of virulence genes in pure bacterial cultures. Their use in complex microbial samples, such as soil and wastewater, has been relatively less studied. This report presents the results of a systematic effort to apply DNA microarrays to pathogen detection and to bacterial source tracking (BST) in wastewater. Parameters such as the method of DNA extraction from the samples, the type of immobilized probe (whether polymerase chain reaction PCR] amplicons or oligonucleotides), the length and method of immobilization of oligonucleotides, the method of DNA labelling, the combination of PCR amplification with microarray hybridization and the choice of PCR targets have been optimized.

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