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Préparation, caractérisation et évaluation in vitro de scaffolds 3D by Paulo A. L. Lima, Gabriela S. Andrade, Luis E. Almeida

Préparation, caractérisation et évaluation in vitro de scaffolds 3D

by Paulo A. L. Lima , Gabriela S. Andrade , Luis E. Almeida
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Book cover type: Paperback
  • ISBN13: 9786209308574
  • Binding: Paperback
  • Subject: N/A
  • Publisher: Editions Notre Savoir
  • Publisher Imprint: Editions Notre Savoir
  • Publication Date:
  • Pages: 64
  • Original Price: GBP 34.71
  • Language: French
  • Edition: N/A
  • Item Weight: 100 grams
  • BISAC Subject(s): General

Les �chafaudages 3D en chitosane (CHI), fibro�ne (SF) et hydroxyapatite (HA) sont biocompatibles et int�ressants pour des applications dans l'ing�nierie tissulaire osseuse. L'objectif �tait donc de pr�parer, caract�riser et �valuer le comportement in vitro des �chafaudages 3D en CHI, CHI/SF, CHI/SF/HA. Les biomat�riaux ont �t� produits � partir d'une solution de CHI, CHI/SF et CHI/SF/HA dans de l'acide ac�tique et, apr�s les processus de production, ils ont �t� soumis � des tests in vitro avec des cellules SaOS-2 pendant 7, 14 et 21 jours. Les �chafaudages ont �t� caract�ris�s par diffraction des rayons X, spectroscopie infrarouge � transform�e de Fourier avec r�flectance totale att�nu�e, analyse thermogravim�trique, calorim�trie diff�rentielle exploratoire, microscopie �lectronique � balayage, spectroscopie � �nergie dispersive, et soumis � des tests pour v�rifier la porosit�, la taille des pores et l'angle de contact. La viabilit� et l'activit� cellulaire ont �t� �valu�es par r�duction du MTT et par d�tection de l'activit� de la phosphatase alcaline. Les �chafaudages 3D ont �t� consid�r�s comme non toxiques et capables de favoriser l'adh�sion, la prolif�ration et la diff�renciation cellulaires, et peuvent �tre utilis�s dans le traitement de la r�paration des d�fauts osseux.

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