Analisi molecolare dei rizobatteri diazotrofi nel riso
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Due variet� di riso tradizionali, Sataria e Kartki, sono state raccolte da campi specifici situati nel distretto di Madhubani, nel Bihar settentrionale. Un'ampia gamma di batteri azotofissatori (diazotrofi) � stata isolata dalle radici di queste due variet� di riso mediante arricchimento in terreni semisolidi privi di azoto;Utilizzando sonde oligonucleotidiche dirette all'RNA ribosomiale, 53 isolati da Sataria e 137 isolati dalla variet� di riso Kartki sono stati identificati come eubatteri. Di questi 190 isolati, la maggior parte (183) sono stati identificati come proteobatteri α-, β- o γ-. Utilizzando sonde oligonucleotidiche specifiche per il gruppo, 14 isolati sono stati assegnati all'α-, 137 al β- e 32 al sottogruppo γ dei proteobatteri. Cinque diversi rizobatteri endofiti diazotrofi (Herbaspirillum seropedica, Gluconobacter diazotrophicus, Azospirillum brasilense, Burkholderia cepacia e Pseudomonas sp.) sono stati inoculati su piantine di riso Kartki cresciute per via assenica e ne � stato analizzato l'impatto sulla crescita e sulla resa. Tra i cinque batteri diazotrofi, B. cepacia ha mostrato il pi� alto incremento nella crescita delle piante e nella resa in grani.
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