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Calcium Techniques: A Laboratory Manual by Jan B. Parys, Martin Bootman, David I. Yule

Calcium Techniques: A Laboratory Manual

by Jan B. Parys , Martin Bootman , David I. Yule
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Book cover type: Paperback
  • ISBN13: 9781936113583
  • Binding: Paperback
  • Subject: N/A
  • Publisher: Cold Spring Harbor Laboratory Press
  • Publisher Imprint: Cold Spring Harbor Laboratory Press
  • Publication Date:
  • Pages: 608
  • Original Price: USD 28.0
  • Language: English
  • Edition: Lab Manual
  • Item Weight: 1588 grams
  • BISAC Subject(s): Life Sciences / Biochemistry

Life begins with a surge of calcium ions (Ca2+) at fertilization, and thereafter, Ca2+ signaling influences nearly every aspect of mammalian development and physiology, from gene expression and cell proliferation to muscle contraction and nerve impulses. To create spatiotemporally distinct Ca2+ signals, cells use a variety of mechanisms to recognize, transport, and buffer Ca2+. Thus, a diverse range of reliable experimental techniques is necessary to study the movement of Ca2+ and the various effectors involved.

This laboratory manual provides step-by-step protocols for studying many facets of Ca2+ signaling, as well as background information on the principles and applications of the techniques. Contributors discuss how to use fluorescent, luminescent, and genetically encoded Ca2+ probes in conjunction with state-of-the-art imaging modalities to characterize Ca2+ signals. Electrophysiological measurements of Ca2+ channel activity are described, as are radioactive Ca2+ flux assays and methods to investigate signaling mediated by specific Ca2+-mobilizing messengers (IP3, cADPR, and NAADP). Techniques to modulate and suppress intra- and intercellular signals are also provided. Each protocol is complete with a list of required materials, detailed recipes for media and reagents, and troubleshooting advice.

Specific chapters are devoted to Ca2+ signaling techniques in non-mammalian systems, such as plants, yeast, zebrafish, and Xenopus. Methods for assessing Ca2+-binding kinetics and strategies for developing mathematical models of Ca2+ signaling are also included. Thus, this manual is a comprehensive laboratory resource for biochemists, cell and developmental biologists, and physiologists who are using or looking to expand their repertoire of Ca2+ techniques.

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