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Caracterisation des cellules mesenchymateuses non manipulees by Dupre-N

Caracterisation des cellules mesenchymateuses non manipulees

by Dupre-N
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Book cover type: Paperback
  • ISBN13: 9786131527647
  • Binding: Paperback
  • Subject: N/A
  • Publisher: Univ Europeenne
  • Publisher Imprint: Univ Europeenne
  • Publication Date:
  • Pages: 88
  • Original Price: GBP 33.0
  • Language: French
  • Edition: N/A
  • Item Weight: 141 grams
  • BISAC Subject(s): Life Sciences / Biology

Les cellules m�senchymateuses (CM) ouvrent de s�duisantes perspectives pour de nouvelles applications en th�rapie cellulaire. Leur raret� dans la moelle osseuse implique une s�lection par adh�rence et une expansion in vitro syst�matique. Celle-ci s'accompagne d'une homog�n�isation de leurs caract�ristiques biologiques laissant supposer que les CM amplifi�es ne sont peut-�tre pas identiques aux CM natives. Notre travail montre que tous les CFU-F sont inclus dans la population des cellules adh�rentes CD45-CD14-/GlyA-, ce qui conforte la n�gativit� de l'antig�ne CD45 sur les CM. En fait, deux sous populations: CD45-CD14- /CD73+ et CD45-CD14-/CD49a+, isol�es de moelle fra�che, contiennent l'une et l'autre l'ensemble des prog�niteurs m�senchymateux. Quelques diff�rences apparaissent entre CM natives et expandues sugg�rant une modification in vitro de l'expression de certains antig�nes. L'ensemble de ces r�sultats nous conduit � proposer un ph�notypage CD45-CD14-/CD73+ et un test fonctionnel CFU-F pour le Contr�le de Qualit� des cellules m�senchymateuses utilis�es en th�rapie cellulaire, notamment en orthop�die et dans les allogreffes.

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