Caratterizzazione molecolare dei batteri produttori di polidrossibutirrato

Name: Caratterizzazione molecolare dei batteri produttori di polidrossibutirrato
Brand: Edizioni Sapienza
SKU: 9786209995095
Price: 5319.0 INR
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ISBN13: 9786209995095
Binding: Paperback
Subject: N/A
Publisher: Edizioni Sapienza
Publisher Imprint: Edizioni Sapienza
Publication Date: May 13, 2026
Pages: 76
Original Price: GBP 42.0
Language: Italian
Edition: N/A
Item Weight: 114 grams
BISAC Subject(s): Life Sciences / Microbiology

About The Book

I polidrossibutirrati sono biopolimeri che presentano il potenziale per essere utilizzati come plastiche biodegradabili in sostituzione delle plastiche sintetiche. La presente indagine � stata condotta per isolare i batteri produttori di polidrossibutirrato e analizzare la diversit� tra essi in relazione alla sequenza del gene della PHB sintasi. I campioni di suolo e acqua utilizzati per l'isolamento dei batteri sono stati raccolti da quattordici fonti diverse. Su un centinaio di isolati batterici, quaranta sono risultati produttori di PHB dopo uno screening iniziale basato sulla fluorescenza su un terreno contenente blu del Nilo A. Provando diverse combinazioni di fonti di carbonio e azoto, sono stati individuati come pi� adatti 2 g di glucosio come fonte di carbonio e 0,65 g di nitrato di potassio. Sono stati selezionati i sei ceppi migliori per l'accumulo di PHB e caratterizzati per l'ottimizzazione dei terreni di produzione di PHB. I ceppi selezionati producevano il 59-29% di PHB rispetto al peso secco del pellet cellulare. Sono state provate diverse combinazioni di sottoprodotti agricoli come fonte di carbonio per una produzione di PHB economicamente vantaggiosa e si � riscontrato che 3 g di melassa costituivano la migliore fonte di carbonio per la produzione di PHB (60% del peso secco delle cellule). L'amplificazione PCR � stata eseguita mediante primer specifici per il gene della PHB sintasi con DNA genomico dei ceppi selezionati.

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