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Clonazione molecolare del gene della tossina emolisi alfa dello Staphylococcus Aureus by Darwesh, Nada

Clonazione molecolare del gene della tossina emolisi alfa dello Staphylococcus Aureus

by Nada Darwesh , Gamal Abdel-Fattah , Elsayed Hafez
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Book cover type: Paperback
  • ISBN13: 9786208035877
  • Binding: Paperback
  • Subject: N/A
  • Publisher: Edizioni Sapienza
  • Publisher Imprint: Edizioni Sapienza
  • Publication Date:
  • Pages: 100
  • Original Price: GBP 42.0
  • Language: Italian
  • Edition: N/A
  • Item Weight: 159 grams
  • BISAC Subject(s): Life Sciences / Genetics & Genomics

La tecnologia ricombinante ha un impatto cruciale nello sviluppo di terapie. Nell'ambiente microbico, i batteri patogeni come lo Staphylococcus aureus producono la proteina alfa-emolisina. Il gene dell'emolisina alfa è stato isolato da S. aureus patogeno isolato da campioni di sangue di pazienti (bambini). Questo gene hia ricombinante è stato prodotto come proteina alfa-emolisina utilizzando E. coli competente come ospite. La proteina α-tossina ricombinante è stata utilizzata come agente antitumorale. In questo studio, l'alta concentrazione di α-tossina (500 μl/ml) ha avuto effetti sulla vitalità cellulare di quattro linee cellulari (HepG-2, HCT-116, MCF-7, A-549). La vitalità cellulare delle linee cellulari era rappresentata dal 31,69% in HepG-2, 36,27% in HCT-116, 78,95% in MCF-7, 67,28% in A-549. Dai nostri risultati, la proteina alfa-emolisina ha avuto un'elevata attività inibitoria nei confronti di HepG-2 e HCT-116, e una debole attività inibitoria nei confronti di MCF-7 e A-549. In conclusione, la produzione della tossina α ricombinante è stata un processo molto semplice, a basso costo, ad alta efficienza, non tossico, ad alta resa e senza effetti collaterali.

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