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Compétence de développement des ovocytes de chèvre maturés in vitro by Singh, Kaushlendra Pratap

Compétence de développement des ovocytes de chèvre maturés in vitro

by Kaushlendra Pratap Singh , Atul Saxena S. D. Kharche
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Book cover type: Paperback
  • ISBN13: 9786208862633
  • Binding: Paperback
  • Subject: N/A
  • Publisher: Editions Notre Savoir
  • Publisher Imprint: Editions Notre Savoir
  • Publication Date:
  • Pages: 100
  • Original Price: GBP 43.41
  • Language: French
  • Edition: N/A
  • Item Weight: 146 grams
  • BISAC Subject(s): Veterinary Medicine / General

La production d'embryons in vitro constitue une excellente source d'embryons pour la recherche fondamentale sur la physiologie du d�veloppement, l'�levage d'animaux de ferme et l'application commerciale des biotechniques �mergentes telles que le sexage des embryons, l'injection de sperme, le transfert nucl�aire, le clonage et la transg�n�se. La technique de la maturation in vitro, de la f�condation et de la culture d'embryons implique essentiellement la collecte d'ovocytes � partir d'ovaires d'abattoirs ou d'animaux vivants par aspiration transvaginale, la maturation in vitro d'ovocytes s�lectionn�s dans des milieux contenant du s�rum et des hormones, la f�condation in vitro avec du sperme congel� et d�congel� ou des spermatozo�des fra�chement collect�s et capacit�s in vitro et la culture in vitro d'embryons dans des milieux contenant des cellules de l'oviducte ou du cumulus jusqu'au stade de la morula ou du blastocyste, stade auquel ils peuvent �tre soit transf�r�s � des receveuses synchronis�es, soit congel�s et conserv�s. La pr�sente �tude vise � �valuer les effets de diff�rentes suppl�mentations dans le milieu de base sur la maturation in vitro et le taux de clivage et � �tudier la comp�tence de d�veloppement des ovocytes de ch�vre f�cond�s in vitro sur des monocouches de cellules de la granulosa et des cellules �pith�liales de l'oviducte en co-culture.

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