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Computational Methods for Fragment Spectrum Interpretation by Jonathan Rameseder, Lennart Martens

Computational Methods for Fragment Spectrum Interpretation

by Jonathan Rameseder , Lennart Martens
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Book cover type: Paperback
  • ISBN13: 9783639228588
  • Binding: Paperback
  • Subject: N/A
  • Publisher: VDM Verlag
  • Publisher Imprint: VDM Verlag
  • Publication Date:
  • Pages: 56
  • Original Price: GBP 40.67
  • Language: English
  • Edition: N/A
  • Item Weight: 96 grams
  • BISAC Subject(s): Life Sciences / Biology

Post-translational modifications (PTMs) are of critical importance for a vast range of cellular processes such as growth control and signal transduction. Mass spectrometry (MS) can shed light on the characteristics of PTMs to gain insight into the mechanisms of numerous diseases including cancer. However, interpretation of tandem MS spectra including fragment ions with PTMs from high-throughput experiments is a complex challenge. The high number and diversity of known PTMs as well as suboptimal quality of spectra compromise the identification of PTMs in MS experiments. In this book, an efficient algorithm for the computational interpretation of tandem MS spectra including fragment ions with PTMs is described. Information on both precursor and fragment ion level is utilized and an arbitrary number of PTMs is considered. A robust statistical noise intensity model is developed to compensate for low spectrum quality. Finally, the algorithm is successfully applied on the fragment spectra in the PRIDE proteomics data repository to render it suitable for statistical machine learning investigations.

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