Confocal and Two-Photon Microscopy
Foundations, Applications, and Advances
Edited by Alberto Diaspro
Confocal and two-photon fluorescence microscopy has provided researchers with unique possibilities of three-dimensional imaging of biological cells and tissues and of other structures such as semiconductor integrated circuits. Confocal and Two-Photon Microscopy: Foundations, Applications, and Advances provides clear, comprehensive coverage of basic foundations, modern applications, and groundbreaking new research developments made in this important area of microscopy.
Opening with a foreword by G. J. Brakenhoff, this reference gathers the work of an international group of renowned experts in chapters that are logically divided into balanced sections covering theory, techniques, applications, and advances, featuring:
* In-depth discussion of applications for biology, medicine, physics, engineering, and chemistry, including industrial applications
* Guidance on new and emerging imaging technology, developmental trends, and fluorescent molecules
* Uniform organization and review-style presentation of chapters, with an introduction, historical overview, methodology, practical tips, applications, future directions, chapter summary, and bibliographical references
* Companion FTP site with full-color photographs
* The significant experience of pioneers, leaders, and emerging scientists in the field of confocal and two-photon excitation microscopy
Confocal and Two-Photon Microscopy: Foundations, Applications, and Advances is invaluable to researchers in the biological sciences, tissue and cellular engineering, biophysics, bioengineering, physics of matter, and medicine, who use these techniques or are involved in developing new commercial instruments.
Alberto Diaspro was born in Genoa, Italy, on April 7, 1959, and went on to receive his doctoral degree in Electronic Engineering from the University of Genoa in 1983. He is an investigator and professor for the Department of Physics at the University of Genoa, and a member of the National Institute for the Physics of Matter (INFM). Dr. Diaspro also holds positions on the editorial boards of several international journals, namely: Microscopy Research and Technique, Journal of Computer Assisted Microscopy, Journal of Biomedical Optics and European Journal of Histochemistry, and he is the author of more than 100 international scientific publications. His main research activities aim at the study of biostructures both in situ and in vitro, but his many interests include the design, realization and utilization of biophysical instrumentation as conventional and confocal microscopy, two-photon fluorescence microscopy and spectroscopy architecture, differential scanning calorimetry, scanning probe microscopy (STM, SNOM, AFM), polarized light scattering, and signal and image digital processing.
