Die Tierische Zelle in Zellkultur
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Die ersten erfolgreichen Versuche zur Z�chtung tierischer Zellen au erhalb des Organismus reichen zur�ck in den Beginn unseres Jahrhunderts. 1907 gelang HARRI- SON durch Explantation von Gewebsst�cken aus Froschembryonen der Nachweis, da in der Embryonalentwicklung die Neuriten als Forts�tze der Nervenzelle ohne Be- teiligung anderer Zellen gebildet werden, und da damit eine spezifische cellul�re Funktion auch au erhalb des Gesamtorganismus zu beobachten ist [310,311]. Au er- dem konnte CARREL 1912 zeigen, da es bei Verwendung einer geeigneten N�hrl�- sung und deren regelm� iger Erneuerung m�glich ist, Kulturen tierischer Gewebe beliebig lange im Zustand aktiver Proliferation und Zellvermehrung zu erhalten [76]. Die Methodik CARRELS war derjenigen von HARRISON recht �hnlich: in bei den F�llen wurden die Kulturen durch Einbettung eines kleinen Gewebsst�ckes in ein Gel - im einen Fall bestehend aus einer Mischung von Blutplasma und Embryo- extrakt, im anderen aus Froschlymphe - hergestellt. In der Zielsetzung jedoch unter- scheiden sich die beschriebenen Versuche voneinander in grunds�tzlicher Weise. HARRISON ging es um die Untersuchung einer spezifischen Funktion unter Bedingun- gen in vitro, w�hrend CARRELS Bem�hungen auf die unbeschr�nkte Fortsetzung der Zellvermehrung au erhalb des Gesamtorganismus ausgerichtet waren. Es zeigte sich schon bald, da im allgemeinen Zellfunktion und Zell vermehrung in vitro gewisserma en antagonistische Prozesse darstellen. Nach Explantation eines Gewebsst�ckes in einem Plasma-Gel l� t sich n�mlich nach kurzer Zeit eine zuneh- mende Desorganisation, vor allem an den R�ndern des Gewebsst�ckes, durch Zell- auswanderung und Zellteilung beobachten [520]. Mit dem Verlust der spezifischen Strukturgeht dann meist auch ein Verlust der typischen Zellfunktionen einher.
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