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Effetto del peptide antimicrobico LL-37 contro Pseudomonas areuginosa by Kais Kassim Ghaima

Effetto del peptide antimicrobico LL-37 contro Pseudomonas areuginosa

by Kais Kassim Ghaima , Fadi Salah Al-Sabagh
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Book cover type: Paperback
  • ISBN13: 9786208727963
  • Binding: Paperback
  • Subject: N/A
  • Publisher: Edizioni Sapienza
  • Publisher Imprint: Edizioni Sapienza
  • Publication Date:
  • Pages: 96
  • Original Price: GBP 59.94
  • Language: Italian
  • Edition: N/A
  • Item Weight: 141 grams
  • BISAC Subject(s): Life Sciences / Microbiology

Un totale di 39 isolati di P. aeruginosa � stato utilizzato per rilevare lo sviluppo di biofilm utilizzando il metodo della piastra microtiter; 10 di questi isolati erano isolati clinici con livelli significativi di resistenza agli antibiotici. Le concentrazioni minime inibitorie (MIC) del peptide antimicrobico LL-37 contro otto isolati di P. aeruginosa multiresistenti sono state determinate utilizzando un saggio su piastra microtiter con colorante resazurina. Nel frattempo, le MIC della ciprofloxacina sono state determinate utilizzando gli stessi isolati e lo stesso metodo e i risultati hanno mostrato che l'attivit� inibitoria variava da 7,8 a 125 μg/ml. I risultati dell'analisi dell'effetto sinergico della combinazione di LL-37 e ciprofloxacina con il metodo checkerboard hanno mostrato che le MIC per entrambi i peptidi antimicrobici sono state abbassate a livelli estremamente bassi, con FIC che variavano da (0,0024-0,26 μg/ml).Secondo i nostri risultati, gli isolati locali a valori inferiori alla MIC hanno significativamente down-regolato i geni coinvolti nella formazione del biofilm. Per i sei isolati, il fold dell'espressione del gene lasI con il solo peptide LL-37 variava da 0,18 a 0,97, e si � verificata una down-regulation dell'espressione genica rispetto al controllo.

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