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Einsatz von ultrahellen Leuchtdioden in der digitalen Holographie by Stürwald, Stephan

Einsatz von ultrahellen Leuchtdioden in der digitalen Holographie

by Stephan Stürwald
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Book cover type: Paperback
  • ISBN13: 9783656382812
  • Binding: Paperback
  • Subject: N/A
  • Publisher: Grin Verlag
  • Publisher Imprint: Grin Verlag
  • Publication Date:
  • Pages: 124
  • Original Price: USD 67.5
  • Language: German
  • Edition: N/A
  • Item Weight: 173 grams
  • BISAC Subject(s): Physics / Optics & Light

Diplomarbeit aus dem Jahr 2008 im Fachbereich Physik - Optik, Note: 1,3, Westfälische Wilhelms-Universität Münster (Physik), Sprache: Deutsch, Abstract: Im Vergleich zu anderen Methoden wie z.B. der Fluoreszenzmikroskopie ermöglichen digitalholographische Verfahren eine Detektion von optischen Weglängenänderungen mit interferometrischer Genauigkeit, die im Refexionsfall durch die Form des Objektes und bei Transmission durch dessen Brechungsindex bzw. deren Änderung verursacht werden. In der Lebendzellanalyse erlauben digitalholographische Verfahren eine gleichzeitig schnelle, minimal invasive, fächenhafte, markerfreie und quantitative Analyse von lebenden Zellen. Hierbei werden die durch die Probe verursachten Variationen in der optischen Weglänge aufgezeichnet und als quantitative Phasenkontrastbilder rekonstruiert. Laser führen aufgrund der gro en Kohärenzlänge bei der digitalen Holographie zu störenden zusätzlichen Interferenzen, die z.B. durch Mehrfachrefxionen im Aufbau verursacht werden. Der Einsatz von kurzkohärenten Lichtquellen eröffnet hierbei die Möglichkeit zur Verminderung dieser Eekte. Es ist daher zu erwarten, dass kurzkohärentes Licht zu einem geringeren Phasenrauschen und damit zu einer besseren Qualität des holographischen Phasenkontrastes führt. Ziel dieser Arbeit ist daher der Aufbau, die Optimierung und Charakterisierung eines kurzkohärenten digitalholographischen Mikroskopiesystems, das insbesondere zur Untersuchung von Zellen eingesetzt werden soll. Hierbei werden ultrahelle Leuchtdioden (LEDs) unterschiedlicher Lichtwellenlängen auf die Eignung als Lichtquellen in der digitalen Holographie untersucht, da diese eine Kohärenzlänge von wenigen Mikrometern aufweisen. Zunächst wird hierzu das für die spätere Anwendung wichtige spektrale Verhalten sowie das Abstrahlprofil verschiedener ultraheller LEDs ("Light Emitting Diodes") untersucht. Des Weiteren werden verschiedene Betriebsmodi und Anwendungsmethoden der eingesetzten Lichtquellen erprobt. An

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