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Electron Tomography: Methods for Three-Dimensional Visualization of Structures in the Cell by Joachim Frank

Electron Tomography: Methods for Three-Dimensional Visualization of Structures in the Cell

by Joachim Frank
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Book cover type: Paperback
  • ISBN13: 9781441921727
  • Binding: Paperback
  • Subject: N/A
  • Publisher: Springer
  • Publisher Imprint: Springer
  • Publication Date:
  • Pages: 456
  • Original Price: EUR 229.99
  • Language: English
  • Edition: N/A
  • Item Weight: 686 grams
  • BISAC Subject(s): Electron Microscopes & Microscopy, Materials Science / General, and Life Sciences / Biology

From the Back Cover

Electron tomography has become a standard technique with applications in cell biology, structural biology, and materials science. This definitive work provides a comprehensive treatment of the mathematical background and working methods of three-dimensional reconstruction from tilt series, with special emphasis on the problems presented by limitations of data collection in the transmission electron microscope. In addition to chapters that are applicable to 3D reconstruction in all fields of science, such as radiological imaging in medicine and electron tomography in materials science, Electron Tomography also focuses on specimen preparation and imaging unique to biological electron microscopy.

This extensively revised second edition updates key contributions on the mathematics of 3D reconstruction, and includes new topics such as automated tomography, frozen sectioning of cells, and the interpretation of density maps through methods of fitting, docking, denoising, and segmentation. Each chapter is a self-contained treatise by a world expert in the author's field of research, resulting in an indispensable resource and companion for laboratories that practice electron tomography or seek to implement electron tomography as a tool for visualization of cells and cell components.

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