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Ensaio de fusão de alta resolução para deteção de infecções bacterianas por DST by Kais Ghaima

Ensaio de fusão de alta resolução para deteção de infecções bacterianas por DST

by Kais Ghaima , Riyam Basim
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Book cover type: Paperback
  • ISBN13: 9786208728168
  • Binding: Paperback
  • Subject: N/A
  • Publisher: Edicoes Nosso Conhecimento
  • Publisher Imprint: Edicoes Nosso Conhecimento
  • Publication Date:
  • Pages: 108
  • Original Price: GBP 69.51
  • Language: Portuguese
  • Edition: N/A
  • Item Weight: 155 grams
  • BISAC Subject(s): Life Sciences / Genetics & Genomics

O presente estudo foi realizado para detetar agentes infecciosos sexualmente transmiss�veis (bact�rias e v�rus) atrav�s do desenvolvimento do ensaio HRM (fus�o de alta resolu��o) com PCR multiplex em tempo real utilizando um conjunto de primers de cada agente patog�nico que foi designado por um programa de software profissional (Beacon Designer(TM) automatiza a conce��o de primers e sondas em tempo real) para a dete��o precisa destas infec��es em doentes iraquianos. Este estudo conduziu � dete��o de sete agentes patog�nicos sexualmente transmiss�veis diferentes (3 bact�rias, 3 v�rus e um parasita) entre 150 pacientes suspeitos de serem casais. Os resultados da RT-PCR com ensaio HRM dos controlos positivos demonstraram diferentes curvas de fus�o, em que cada pico de fus�o representa a amlifica��o de um dos sete agentes patog�nicos, bem como o pico de fus�o do controlo interno (gene de manuten��o da casa). Este experimento incluiu tr�s pain�is, o painel um representa o pico de fus�o ideal para o controle positivo de Trichomonas vaginalis, Mycoplasma hominis, Cytomegalovirus, a temperatura de fus�o de cada um deles foi de 80,0 ℃, 84,90 ℃ e 88,0 ℃, respetivamente.

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