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Études Structurales d'Héparanes Sulfates Par Rmn by Pegeot-M

Études Structurales d'Héparanes Sulfates Par Rmn

by Pegeot-M
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Book cover type: Paperback
  • ISBN13: 9783841673077
  • Binding: Paperback
  • Subject: N/A
  • Publisher: Omniscriptum
  • Publisher Imprint: Omniscriptum
  • Publication Date:
  • Pages: 216
  • Original Price: GBP 34.0
  • Language: French
  • Edition: N/A
  • Item Weight: 323 grams
  • BISAC Subject(s): Life Sciences / Biology

Les héparanes sulfates (HS) font partie de la famille des glycosaminoglycanes (GAG) qui sont des polysaccharides linéaires largement retrouvés chez les vertébrés. Selon le tissu et la nature de la protéine coeur sur laquelle les HS sont attachés, le squelette carboné composé - d'un enchainement d'acides glucuroniques (GlcA) et de N-acétylglucosamines (GlcNAc) répétés alternativement - va pouvoir subir différentes modifications. Ces chaines ainsi modifiées vont ètre capables d'interagir avec un très grand nombre de partenaires et jouer des rôles cruciaux dans divers évènements physiologiques (croissance, réponse immunitaire...) et pathologiques (cancer, attachement viral...). Dans ce travail, un protocole de marquage et d'analyse des HS basé sur la résonance magnétique nucléaire (RMN) a été développé afin de déterminer les caractéristiques structurales (modifications, longueur des chaines) de ces polysaccharides marqués issus de cellules de mammifères cultivées in vitro. Cet ouvrage s'adresse aussi bien aux glycobiologistes confirmés qu'aux étudiants scientifiques curieux et désireux d'en apprendre plus sur les HS ainsi que sur leurs techniques d'analyses.

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