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Gene Transfer: Delivery and Expression of DNA and Rna, a Laboratory Manual by Theodore Friedmann, John Rossi

Gene Transfer: Delivery and Expression of DNA and Rna, a Laboratory Manual

by Theodore Friedmann , John Rossi
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Book cover type: Hardcover
  • ISBN13: 9780879697648
  • Binding: Hardcover
  • Subject: N/A
  • Publisher: Cold Spring Harbor Laboratory Press
  • Publisher Imprint: Cold Spring Harbor Laboratory Press
  • Publication Date:
  • Pages: 793
  • Original Price: GBP 164.0
  • Language: English
  • Edition: 1
  • Item Weight: 2495 grams
  • BISAC Subject(s): Life Sciences / Biochemistry

Understanding gene function and regulation requires rigorous testing in live cells and organisms. Recent advances have provided a variety of new strategies for delivering DNA and RNA into cells and probing their expression, as well as new clinical applications that rely upon the introduction of genetic material. The vast number of available techniques for clinical and laboratory research often makes selecting the optimal method a difficult process. Gene Transfer: Delivery and Expression of DNA and RNA provides the first comprehensive guide to technical approaches for delivering nucleic acids into cells and organisms and of ensuring (even manipulating) appropriate expression. The detailed, step-by-step protocols cover a variety of methods, both well established and newly evolving. These include viral and nonviral methods of gene delivery, transgenic approaches, strategies for the regulation of transgene expression, and modification of the host response. The introductory matter to each chapter includes concise technical and theoretical discussions with considerations for selection of the appropriate system and strategies for delivery.

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