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Genetic Library Construction and Screening: Advanced Techniques and Applications by R. C. Bird

Genetic Library Construction and Screening: Advanced Techniques and Applications

by R. C. Bird
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Book cover type: Paperback
  • ISBN13: 9783642477331
  • Binding: Paperback
  • Subject: N/A
  • Publisher: Springer
  • Publisher Imprint: Springer
  • Publication Date:
  • Pages: 241
  • Original Price: EUR 49.99
  • Language: English
  • Edition: 2002
  • Item Weight: 392 grams
  • BISAC Subject(s): Laboratory Medicine, Life Sciences / Molecular Biology, and Life Sciences / Biochemistry

Designed as an introductory text the authors cover all core strategies in the application of modern recombinant DNA technology. The first chapters directly address the applications of polymerase chain reaction to a variety of problems in DNA cloning that are, or have been, extremely challenging using more traditional approaches and technologies. These include cDNA cloning and transcript mapping, mutagenesis as well as the cloning of very long transcripts and protocols using limiting amounts of total RNA. Further chapters describe approaches to subtractive cloning technologies as well as novel specialized expression cloning and library screening strategies. The handbook contains detailed step-by-step protocols and extensive hands-on advice.

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