HPLC-UV Methodenentwicklung für pharmazeutische Formulierungen mit fester Dosierung

Name: HPLC-UV Methodenentwicklung für pharmazeutische Formulierungen mit fester Dosierung
Brand: Verlag Unser Wissen
SKU: 9786209842870
Price: 5113.0 INR
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by Sonia Yadav , Anunny Sharma , Sushma Maratha

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ISBN13: 9786209842870
Binding: Paperback
Subject: N/A
Publisher: Verlag Unser Wissen
Publisher Imprint: Verlag Unser Wissen
Publication Date: April 2, 2026
Pages: 56
Original Price: USD 57.0
Language: German
Edition: N/A
Item Weight: 87 grams
BISAC Subject(s): Pharmacology

About The Book

Sch�tzung von Imegliminhydrochlorid in menschlichem Plasma mittels HPLC-UV. Die Bestimmung von Arzneimitteln mittels RP-HPLC (Umkehrphasenchromatographie) wurde gew�hlt, da sie f�r ionische und leicht bis unpolare Verbindungen geeignet ist. Die Umkehrphasenchromatographie ist einfach, spezifisch und in Bezug auf Effizienz, Stabilit�t und Reproduzierbarkeit �berlegen. F�r die Trennung von Imeglimin wurde die C18-S�ule 250 x 4,6 mm, 5 m Partikelgr� e gew�hlt. F�r die mobile Phase wurden verschiedene L�sungsmittell�sungen getestet und f�r eine optimale Leistung kombiniert. Imeglimin-Hydrochlorid (4 g/ml) in Puffer, pH 4 f�r Acetonitril: Wasser (70:30), ein Konzentrationsbereich (8-40 und 0,5-4 g/ml), der ausgezeichnete Peaks und ein betr�chtliches Aufl�sungsniveau aufweist. Die mobile Phase wurde mit einer Geschwindigkeit von 1 ml/Min. gefahren und Photodiodenarray-Detektoren wurden zum Nachweis beider Analyten bei 215 nm verwendet. Die Methode wurde in verschiedenen Konzentrationsbereichen in Humanplasma validiert. Die Inter- und Intra-Run-Pr�zision von IMEGLIM wurde mit weniger als 3,60 % gemessen und hatte eine Genauigkeit von weniger als 1,869 %. IMEGLIM in Humanplasma wurde durch Linearit�t, Wiederfindungsmatrix und Stabilit�t validiert. Die Retentionszeit von IMEG betr�gt 4,8 Minuten im Plasma. Die vorgeschlagene Methode ist kosteneffektiv.

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