Influence des hydroperoxydes d acides gras sur la yarrowia lipolytica
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Dans le but de produire des composés d'arôme, les hydroperoxydes d'acides gras sont utilisés comme substrats pour la Yarrowia lipolytica exprimant l'hydroperoxyde lyase du poivron vert. Cette enzyme permet la transformation de ces hydroperoxydes en aldéhydes en C6, composés participant aux "notes vertes". La présence des hydroperoxydes dans le milieu de culture a des répercussions sur les levures en inhibant la croissance des cellules, en induisant la chute de leur viabilité et en fluidifiant la membrane cellulaire. Ils sont également à l'origine d'un stress oxydatif sur le métabolisme des cellules de levure. Le potentiel d'oxydo-réduction du milieu semble influer l'impact des hydroperoxydes sur les cellules de levure. Les variations d'activité des enzymes antioxydantes des cellules cultivées dans le milieu réducteur sont moins importantes que celles des cellules cultivées dans le milieu témoin. Un plan d'expérience factoriel a permis de déterminer les facteurs favorisant la production d'hexanal. Il s'agit principalement des substrats hydroperoxydes et de l'agent réducteur. Les conditions optimales de la bioconversion ont ainsi été déterminées.
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