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Interactions faibles entre protéines en solution by Costenaro-L

Interactions faibles entre protéines en solution

by Costenaro-L
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Book cover type: Paperback
  • ISBN13: 9786131506048
  • Binding: Paperback
  • Subject: N/A
  • Publisher: Univ Europeenne
  • Publisher Imprint: Univ Europeenne
  • Publication Date:
  • Pages: 208
  • Original Price: GBP 56.0
  • Language: French
  • Edition: N/A
  • Item Weight: 313 grams
  • BISAC Subject(s): Life Sciences / Biophysics

La cellule est un milieu tr�s concentr� o� les interactions entre macromol�cules, m�me faibles, sont cruciales pour leur solubilit� et leur fonction biologique. Dans quelle mesure la solvatation d''une prot�ine influence-t-elle ses interactions faibles prot�ine - prot�ine, qui d�terminent sa solubilit�? Nous avons reli� ces interactions � la solvatation de la malate d�shydrog�nase de Haloarcula marismortui, une prot�ine halophile dont la solvatation d�pend fortement du solvant. Les interactions prot�ine - prot�ine dans divers solvants ont �t� caract�ris�es en mesurant les seconds coefficients du viriel (A2) et en mod�lisant par des potentiels de type DLVO les facteurs de structure en solution obtenus par la diffusion de rayons X ou de neutrons. Nous avons aussi d�velopp� une nouvelle m�thode de mesure de A2 par vitesse de s�dimentation. Nous montrons qu''une solvatation enrichie en eau ou en sel induit des interactions attractives substantielles qui peuvent conduire � la cristallisation des prot�ines. Ces r�sultats expliquent l''adaptation mol�culaire aux fortes salinit�s des prot�ines halophiles en termes de stabilit�, mais aussi -de mani�re originale- en termes de solubilit�.

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