Kapillarelektrophorese: Methoden Und Möglichkeiten

Name: Kapillarelektrophorese: Methoden Und Möglichkeiten
Brand: Springer
SKU: 9783642633959
Price: 3776.0 INR
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by Heinz Engelhardt , Wolfgang Beck , Thomas Schmitt

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ISBN13: 9783642633959
Binding: Paperback
Subject: N/A
Publisher: Springer
Publisher Imprint: Springer
Publication Date: November 6, 2012
Pages: 206
Original Price: EUR 51.39
Language: German
Edition: Softcover Repri
Item Weight: 313 grams
BISAC Subject(s): Chemistry / Analytic

About The Book

Die Kapillarelektrophorese (CE), auch mit dem Acronym HPCE fiir "High Perfor- mance Capillary Electrophoresis" bezeichnet, ist ein mit hoher Geschwindigkeit wach- sendes instrumentell - analytisches Trennverfahren. Vereint sie doch die Trenntechnik der klassischen Elektrophorese auf Platten mit den instrumentellen Methoden der Chromatographie hinsichtlich direkter Detektion der getrennten Proben in der Kapil- lare, und damit einfach durchzufiihrende Identifizierung und Quantifizierung der Analyten. Die anfiinglichen Probleme mit mangelnder Reproduzierbarkeit der quanti- tativen Analyse, bedingt durch die Notwendigkeit mit extrem kleinen Volumina umzu- gehen, sind bei kommerziellen Geraten der mittlerweile zweiten Generation weitestge- hend gelOst, so daB fiir ionische Verbindungen yom kleinsten Kation (dem Lithium- ion) bis hin zu den Polyanionen mit Molekulargewichten im Millionenbereich (wie DNA-Molekiile) ein schnelles und zuverlassiges Trennverfahren zur Verfiigung steht. Die Methoden der Gelelektrophorese, der isoelektrischen Fokussierung, sind einfach auf die Trenntechnik in der Kapillare zu iibertragen. Fiir nichtionische Verbindungen steht dariiberhinaus ein zusatzliches Trennverfahren in Form der micellaren elektro- kinetischen Chromatographie (MEKC) zur Verfiigung. Hierbei handelt es sich urn eine echte chromatographische Trenntechnik, da der elektrophoretischen Migration die Verteilung der Analyten zwischen dem Puffer und den Micellen iiberlagert ist und dies wesentlich zur Selektivitat beitragt.

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