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Konditionale gezielte Mutagenese in eukaryontischen Zellen by Saße, Alexa

Konditionale gezielte Mutagenese in eukaryontischen Zellen

by Alexa Saße
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Book cover type: Paperback
  • ISBN13: 9783638755429
  • Binding: Paperback
  • Subject: N/A
  • Publisher: Grin Verlag
  • Publisher Imprint: Grin Verlag
  • Publication Date:
  • Pages: 20
  • Original Price: USD 22.5
  • Language: German
  • Edition: N/A
  • Item Weight: 41 grams
  • BISAC Subject(s): Life Sciences / Genetics & Genomics

Praktikumsbericht / -arbeit aus dem Jahr 2004 im Fachbereich Biologie - Genetik / Gentechnologie, Note: "keine", Universität zu Köln (Biologisches Institut), Sprache: Deutsch, Abstract: In diesem ersten Praktikumsteil soll das Prinzip der konditional gezielten Mutagenese in eukaryotischen Zellen vorgestellt werden. Unter einer Mutagenese versteht man die induzierte Mutationsauslösung. Eine Analyse von Mutanten ermöglicht wichtige Einblicke in den Bau und die Funktion von Genen. Das übliche Vorgehen ist die Beschreibung der Auswirkungen von Mutationen auf Reaktionen der betroffenen Zellen oder des betroffenen Organismus. Daraus folgt dann die Isolierung des Gens mit der Bestimmung seiner Struktur durch Nucleotid-Sequenzierung. In diesem Versuchsteil wird die Methode des Conditional Gene Targeting benutzt, mit der es möglich ist, in der Maus gezielt zeit- und gewebespezifisch Gene auszuschalten. So eine Inaktivierung bildet sozusagen einen Funktionstest für das entsprechende Gen.

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