Linear Dichroism and Circular Dichroism: A Textbook on Polarized-Light Spectroscopy

Name: Linear Dichroism and Circular Dichroism: A Textbook on Polarized-Light Spectroscopy
Brand: Royal Society of Chemistry
SKU: 9781847559029
Price: 5712.0 INR
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by Bengt Nordén , Alison Rodger , Tim Dafforn

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ISBN13: 9781847559029
Binding: Hardcover
Subject: N/A
Publisher: Royal Society of Chemistry
Publisher Imprint: Royal Society of Chemistry
Publication Date: September 2, 2010
Pages: 304
Original Price: GBP 39.95
Language: English
Edition: N/A
Item Weight: 794 grams
BISAC Subject(s): Chemistry / Physical & Theoretical

About The Book

This book provides an introduction to optical anisotropy (linear dichroism, LD) and optical activity (circular dichroism, CD) as techniques for the study of structures and interactions of molecules in solution. The book covers the use of these techniques for both small and large molecular systems with particular emphasis being placed on proteins and nucleic acids. CD is a well-established technique and this book aims to explain how it can be used simply and effectively for new entrants to the field as well as covering more advanced techniques for experts. LD is often seen as a rather exotic method intended only for experienced spectroscopists. This book demonstrates that it is an approach with real utility that may be used by both students and scientists from graduate level onwards to give simple answers, which are not available from any other technique, to structural and kinetic questions. Much of the emphasis is on flow orientation of samples in solution phase. The book first describes the techniques and the information they can provide; it then goes on to give specific details on how to actually implement them, including a wide range of examples showing how LD and CD can help with * protein and nucleic acid secondary structure elucidation; * analysis of the formation and rearrangements of fibrous proteins and membrane proteins; * identification of the absolute configuration of small molecules; * determination of the orientation of small molecules in anisotropic media; * assignment of transition moment polarizations; * investigation of binding strengths and geometries of ligand-macromolecule complexes; * 3-D structure determination from LD, molecular replacement and MD modeling. The advantages of combined LD/CD studies are also outlined with examples of DNA/drug complexes and protein insertion into membranes. Taken together the book represents a comprehensive text on the theory and application of LD and CD in the chemical and biological sciences.

About The Author

Bengt NordÚn is a Professor of Physical Chemistry at the Chalmers University of Technology in Sweden. He has a BSc and PhD from the University of Lund and is renowned for pioneering linear dichroism spectroscopy for the study of transition moments, molecular interactions and macromolecular structures in solution. Alison Rodger is a Professor of Biophysical Chemistry in the Department of Chemistry at the University of Warwick. She has a BSc and a PhD from the University of Sydney and an MA from the University of Oxford. Timothy Dafforn has a BSc from the University of Cardiff and a PhD from the University of Bristol. He is a Lecturer at the University of Birmingham and a pioneer in the use of linear dichroism methods in the study of biological systems.

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