Microscopie non linéaire: chiralité et génération de second harmonique
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Depuis 1990, la microscopie non lin�aire de fluorescence excit�e � deux photons ou de g�n�ration de second harmonique a insuffl� un nouvel �lan � la microscopie optique. Il est possible de r�aliser de l''imagerie fonctionnelle tridimensionnelle de tissus �pais vivants ou de cellule. Le contraste provient soit de colorants optimis�s pour l''imagerie non lin�aire, soit de prot�ines naturellement pr�sentes dans les tissus comme l''�lastine ou le collag�ne. Ce travail men� au sein du Laboratoire d''Optique et Biosciences � l''�cole polytechnique est enrichi de nombreuses images obtenues par combinaison des signaux de fluorescence � deux photons et de second harmonique. Il montre tout l''int�r�t de cette technique face � l''histologie classique et permet de mettre en �vidence des effets du lindane (un insecticide tr�s r�pandu) sur le syst�me cardio- circulatoire du Rat. Il a �galement �t� possible de mener une �tude nanotoxinologique � partir du signal de second harmonique des filaments de myosine. Il s''agit d''une mesure de contracture au repos de cellules atriales de grenouille soumises � une toxine (la saxitoxine), avec une pr�cision de 20 nm.
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